Journal: BMC Oral Health

Article Title: Targeting CGRP signaling alleviates cancer-associated pain in oral squamous cell carcinoma

doi: 10.1186/s12903-026-08444-x

Figure Lengend Snippet: Analgesic effects of Rimegepant in OSCC-associated pain demonstrated via the unilateral tongue orthotopic transplantation model. A Construction of the unilateral tongue orthotopic transplantation model and drug injection with mechanical threshold measurement protocol. B Following tumor transplantation, the differences in mechanical sensitivity between the transplanted ( n = 14) and non-transplanted ( n = 10) sides were compared using unpaired two-tailed Student’s t test. C Changes in sensitivity 1 h after Rimegepant (50 mg/kg), Rimegepant (10 mg/kg) and Carprofen treatment were performed using one-way ANOVA. Each dataset included results from monitoring over 4 consecutive days ( n = 6–8/group). D Changes in sensitivity following Rimegepant (50 mg/kg) and Tramadol treatment at 1 h were performed using one-way ANOVA. Each dataset included results from monitoring over 4 consecutive days ( n = 6–8/group). E The 4-day trends of facial mechanical sensitivities 24 h after Rimegepant (50 mg/kg) and Tramadol treatment. ( n = 6–8/group). F Changes in sensitivity 1 h after Tramadol, Carprofen treatment and local lingual injection of Rimegepant (10 mg/kg) in CAL27 and SCC25 xenograft models were performed using one-way ANOVA( n = 8). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns P > 0.05

Article Snippet: Human OSCC CAL27 and SCC25 cells were purchased from the American Type Culture Collection (USA).

Techniques: Transplantation Assay, Injection, Two Tailed Test

Journal: Cancer Cell International

Article Title: LIS1 mediated Schwann cell reprogramming enhances perineural invasion by activating the serine/NMDAR/AKT signaling pathway in head and neck squamous carcinoma

doi: 10.1186/s12935-026-04243-0

Figure Lengend Snippet: LIS1 increases the migration, invasion, and PNI capability of HNSCC cells. ( A ) Wound healing assay of LIS1 overexpression or LIS1 knockdown in SCC25 cells. ( B ) Transwell assay of LIS1 overexpression or LIS1 knockdown in SCC25 cells. ( C ) Representative images of the in vitro PNI model for LIS1 overexpression or LIS1 knockdown in SCC25 cells. ( D ) Microfluidic chip pattern diagram and representative images of LIS1 overexpression or LIS1 knockdown in SCC25 cells co-cultured with SCs in the microfluidic chip. Data are represented as mean ± SEM, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Human HNSCC cell line (SCC25) and human Schwann cells (sNF96.2) were acquired from the American Type Culture Collection (ATCC, Manassas, VA, USA) in 2023.

Techniques: Migration, Wound Healing Assay, Over Expression, Knockdown, Transwell Assay, In Vitro, Cell Culture

Journal: Cancer Cell International

Article Title: LIS1 mediated Schwann cell reprogramming enhances perineural invasion by activating the serine/NMDAR/AKT signaling pathway in head and neck squamous carcinoma

doi: 10.1186/s12935-026-04243-0

Figure Lengend Snippet: LIS1 overexpression of HNSCC cells increases Schwann cells activity. ( A ) The pattern diagram of HNSCC cells co-cultured with SCs. ( B ) Transwell assay of sNF96.2 cells co-cultured with SCC25 cells. ( C ) The mRNA expression of PNI-related factors (BDNF, GDNF, NGF, MMP2, and MMP9) in sNF96.2 cells co-cultured with SCC25 cells were analyzed by RT-qPCR. ( D ) IF double staining was performed to detect the expression of S100 and GFAP in the sNF96.2 cells co-cultured with SCC25 cells. Data are represented as mean ± SEM, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Human HNSCC cell line (SCC25) and human Schwann cells (sNF96.2) were acquired from the American Type Culture Collection (ATCC, Manassas, VA, USA) in 2023.

Techniques: Over Expression, Activity Assay, Cell Culture, Transwell Assay, Expressing, Quantitative RT-PCR, Double Staining

Journal: Cancer Cell International

Article Title: LIS1 mediated Schwann cell reprogramming enhances perineural invasion by activating the serine/NMDAR/AKT signaling pathway in head and neck squamous carcinoma

doi: 10.1186/s12935-026-04243-0

Figure Lengend Snippet: LIS1 overexpression activates the serine signaling pathway to promote the PNI in HNSCC cells. ( A ) The mRNA expression of PHGDH, PSAT1, and PSPH in SCC25 cells were analyzed by RT-qPCR. ( B ) Analysis of the relative intracellular serine levels in SCC25 cells. ( C ) The protein level of PHGDH, PSAT1, and PSPH in SCC25 cells were analyzed by WB. ( D ) Migration and invasion assay of shLIS1 group after exogenous serine stimulation of SCC25 cells and quantitative analysis. ( E ) Representative images of the in vitro PNI model for shLIS1 group after exogenous serine stimulation of SCC25 cells and the analysis of neural invasion index. ( F ) Representative images of the microfluidic chip for shLIS1 group after exogenous serine stimulation of SCC25 cells and analysis of migration distance. Data are represented as mean ± SEM, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Human HNSCC cell line (SCC25) and human Schwann cells (sNF96.2) were acquired from the American Type Culture Collection (ATCC, Manassas, VA, USA) in 2023.

Techniques: Over Expression, Expressing, Quantitative RT-PCR, Migration, Invasion Assay, In Vitro